GrainGenes Marker Report: Xmwg502-5R
Locus
Xmwg502-5R
Type
RFLP
Chromosome
5R
Map
Secale-N6xN2-2004-5R 0 Secale-N7xN2-2004-5R 5.6 Secale-N7xN6-2004-5R 0 Secale-PxP-2004-5R 4.1
[ Show Nearby Loci ]
Map Data
Rye, N6 x N2, 2004 Rye, N7 x N2, 2004 Rye, N7 x N6, 2004 Rye, P87 x P105, 2004
Species
Secale cereale
Probe
MWG502
Reference
Khlestkina EK et al. (2004) Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags Theoretical and Applied Genetics 109:725-732.
Possible Orthologs
MWG502 Xmwg502
Probe for Locus
MWG502
Locus
MWG502 Xmwg502 Xmwg502-5R
Reference
General Remarks
Restriction Enzyme for CAPS marker is Msp I; Hpa II
Type
Genomic STS
PCR primers
5' tgtttagctaagctgttgctgagg 3'
5' ctctgaagcatggaaccagatttg 3'
STS primers
T7 side (5' to 3') ATCAAGATGAGGAAGCTGATGCAGT
T3 side (5' to 3') TCTTGTCTGAAGCATGGAACCAGAT
Amplification Conditions
5 min at 94 deg C; 35 cycles with 30 sec at 94 deg C, 30 sec at 55 deg C, and 1 min at 72 deg C; and a final extension step of 7 min at 72 deg C
Sequence
AJ234471 AJ234470 MWG502 MWG502-5 MWG502-3
Linkage Group
5H
Insert Enzyme
PstI
Source Species
Hordeum vulgare
Insert Size
0.9
GrainGenes Marker Report: Xmwg502-5R
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
|