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GrainGenes Marker Report: Xpsp3009-6B

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Locus	Xpsp3009-6B
Type	Microsatellite
Chromosome	6B
Chromosome Arm	6BS
Map	CS/SQ1-6B 49.6 Ta-Gale-6B -19.5
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Map Data	Wheat, Chinese Spring x SQ1 Wheat, Gale
Probe	PSP3009
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark	Quality score = 5 (multiple products and multiple loci, difficult to relate bands between genotypes)
Probe for Locus	PSP3009
Locus	Xpsp3009-6B Xpsp3009
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949. Bryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks	Insert contains repeat (CA)34 (imperfect)
Type	PCR SSR
PCR primers	Forward CACCGGTTTTGACACCGATAGCC Reverse TGGGTAAGCTGCATGGGCAAACC
SSR size	221, 6B locus
Amplification Conditions	63C anneal
Sequence	PSP3009
Source Species	Triticum aestivum