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GrainGenes Marker Report: Xpsp3028-4A

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Locus
Xpsp3028-4A
Type
Microsatellite
Chromosome
4A
Chromosome Arm
4AS
Map
CS/SQ1-4A191.8
Ta-Gale-4A-4.5
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Map Data
Wheat, Chinese Spring x SQ1
Wheat, Gale
Probe
PSP3028
Reference
ReferenceStephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark
Quality score = 2 (single product recognizing a single locus, some stuttering however easy to score)



Probe for Locus
PSP3028
Locus
Xpsp3028-4A
Xpsp3028
Reference
ReferenceStephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
ReferenceBryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks
Insert contains repeat (GA)2d
Type
PCR
SSR
PCR primers
Forward GCTTCATGCATGTGTCAGGTAG
Reverse AGTTGTACGGTCGACTGTCG
SSR size
123, 4A locus
Amplification Conditions
58C anneal
Sequence
PSP3028
Source Species
Triticum aestivum