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GrainGenes Marker Report: Xpsp3050-7A

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Locus
Xpsp3050-7A
Type
Microsatellite
Chromosome
7A
Chromosome Arm
7AS
Map
CS/SQ1-7A111.5
Ta-Courtot/CS-GPW-7A138.4
Ta-Gale-7A-5.6
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Map Data
Wheat, Chinese Spring x SQ1
Wheat, Courtot x Chinese Spring, GPW
Wheat, Gale
Probe
PSP3050
Reference
ReferenceStephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark
Quality score = 2 (single product recognizing a single locus, some stuttering however easy to score)



Probe for Locus
PSP3050
Locus
Xpsp3050-7A
Xpsp3050
Reference
ReferenceStephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
ReferenceBryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks
Insert contains repeat (CT)12(AT)13
Type
PCR
SSR
PCR primers
Forward TAACTCACCTGCGAACTGTG
Reverse CCGATAAAAGTTTAGCGACCC
SSR size
156, 7A locus
Amplification Conditions
58C anneal
Sequence
PSP3050
Source Species
Triticum aestivum