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GrainGenes Marker Report: Xpsp3058-2D

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Locus	Xpsp3058-2D
Type	Microsatellite
Chromosome	2D
Chromosome Arm	2DS
Map	Ta-Gale-2D -4.7
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Map Data	Wheat, Gale
Probe	PSP3058
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark	Quality score = 5 (multiple products and multiple loci, difficult to relate bands between genotypes)
Probe for Locus	PSP3058
Locus	Xpsp3058-6D Xpsp3058-2D Xpsp3058-4A
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949. Bryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks	Insert contains repeat (CCT)3(TCT)12
Type	PCR SSR
PCR primers	Forward GATCGCCGTCCCGATCGTTG Reverse AATCTCGAAGCGCTCCCGCC
SSR size	189, 6D locus
Amplification Conditions	63C anneal
Sequence	PSP3058
Source Species	Triticum aestivum