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GrainGenes Marker Report: Xpsp3065-5B

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Locus	Xpsp3065-5B
Type	Microsatellite
Chromosome	5B
Chromosome Arm	5BL
Map	Ta-Gale-5B 104
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Map Data	Wheat, Gale
Probe	PSP3065
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark	Quality score = 4 (multiple products and multiple loci, easy to score but sometimes difficult to relate polymrphic loci to those previously characterized and mapped)
Probe for Locus	PSP3065
Locus	Xpsp3065-4B Xpsp3065-5B Xpsp3065
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949. Bryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks	Insert contains repeat (CAG)4-(AGG)5
Type	PCR SSR
PCR primers	Forward TGCTCCACGCCGCGCCAC Reverse ACCACCTCGTACGGCCCATC
SSR size	263, 5B locus
Amplification Conditions	65C anneal
Sequence	PSP3065
Source Species	Triticum aestivum