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GrainGenes Marker Report: Xpsp3081-7B

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Locus	Xpsp3081-7B
Type	Microsatellite
Chromosome	7B
Chromosome Arm	7BL
Map	Ta-Courtot/CS-GPW-7B 134.1 Ta-Gale-7B 71
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Map Data	Wheat, Courtot x Chinese Spring, GPW Wheat, Gale
Probe	PSP3081
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark	Quality score = 2 (single product recognizing a single locus, some stuttering however easy to score)
Probe for Locus	PSP3081
Locus	Xpsp3081-3B Xpsp3081-7B Xpsp3081b
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949. Bryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks	Insert contains repeat (TG)12 (imperfect)
Type	PCR SSR
PCR primers	Forward ACCTTAACTAGTTCTTTGGCAAGC Reverse GCATAAGATAGCTTCTGCTCCCTTC
SSR size	139, 7B locus
Amplification Conditions	61C anneal
Sequence	PSP3081
Source Species	Triticum aestivum