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GrainGenes Marker Report: Xpsp3113-7D

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Locus
Xpsp3113-7D
Type
Microsatellite
Chromosome
7D
Chromosome Arm
7DL
Map
CS/SQ1-7D140
Ta-Gale-7D1.7
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Map Data
Wheat, Chinese Spring x SQ1
Wheat, Gale
Probe
PSP3113
Reference
ReferenceStephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark
Quality score = 3 (single locus, profound and/or irregular stuttering, sometimes difficult to score)



Probe for Locus
PSP3113
Locus
Xpsp3113-7D
Xpsp3113
Reference
ReferenceStephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
ReferenceBryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks
Insert contains repeat (CA)17
Type
PCR
SSR
PCR primers
Forward CTCTCAAAGCCGCCACCATAGTC
Reverse CGTCGCTTACCGGTCCTGTCC
SSR size
162, 7D locus
Amplification Conditions
61C anneal
Sequence
PSP3113
Source Species
Triticum aestivum