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GrainGenes Marker Report: Xpsp3114-7A

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Locus	Xpsp3114-7A
Type	Microsatellite
Chromosome	7A
Chromosome Arm	7AS
Map	Ta-Gale-7A -0.6
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Map Data	Wheat, Gale
Probe	PSP3114
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark	Quality score = 2 (single product recognizing a single locus, some stuttering however easy to score)
Probe for Locus	PSP3114
Locus	Xpsp3114-7A
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949. Bryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks	Insert contains repeat (CA)12(imperfect)(TA)4
Type	PCR SSR
PCR primers	Forward ATAATAAGGGGTGGCACTGCC Reverse ATTCAGAATATATTCTTTTACTGCC
SSR size	116, 7A locus
Amplification Conditions	63C anneal
Sequence	PSP3114
Source Species	Triticum aestivum