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GrainGenes Marker Report: Xpsp3131-6B

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Locus	Xpsp3131-6B
Type	Microsatellite
Chromosome	6B
Chromosome Arm	6BL
Map	Ta-Gale-6B 7
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Map Data	Wheat, Gale
Probe	PSP3131
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark	Quality score = 4 (multiple products and multiple loci, easy to score but sometimes difficult to relate polymrphic loci to those previously characterized and mapped)
Probe for Locus	PSP3131
Locus	Xpsp3131-6B Xpsp3131 Xpsp3131-6D
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949. Bryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks	Insert contains repeat (GAA)13 (imperfect)
Type	PCR SSR
PCR primers	Forward GCTAGTCCCGACGCCCTATC Reverse GAGGAAGGAGCTTTGGTTTCTCC
SSR size	144, 6B locus
Amplification Conditions	61C anneal
Sequence	PSP3131
Source Species	Triticum aestivum