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GrainGenes Marker Report: Xpsp3142-2A

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Locus	Xpsp3142-2A
Type	Microsatellite
Chromosome	2A
Chromosome Arm	2AS
Map	Ta-Gale-2A -3.9
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Map Data	Wheat, Gale
Probe	PSP3142
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark	Quality score = 4 (multiple products and multiple loci, easy to score but sometimes difficult to relate polymrphic loci to those previously characterized and mapped)
Probe for Locus	PSP3142
Locus	Xpsp3142-2A Xpsp3142-4A
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949. Bryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks	Insert contains repeat (CAA)12+
Type	PCR SSR
PCR primers	Forward AGATACGTTGGAGACGTATCACGC Reverse CATCCAGTTGATGTGTGGCAAAGC
SSR size	645, 2A locus
Amplification Conditions	61C anneal
Sequence	PSP3142
Source Species	Triticum aestivum