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GrainGenes Marker Report: Xpsp3144-3B

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Locus	Xpsp3144-3B
Type	Microsatellite
Chromosome	3B
Chromosome Arm	3BL
Map	CS/SQ1-3B 102.5 Ta-Gale-3B 4.9
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Map Data	Wheat, Chinese Spring x SQ1 Wheat, Gale
Probe	PSP3144
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark	Quality score = 2 (single product recognizing a single locus, some stuttering however easy to score)
Probe for Locus	PSP3144
Locus	Xpsp3144-3B Xpsp3144
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949. Bryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks	Insert contains repeat (TC)12 (imperfect)
Type	PCR SSR
PCR primers	Forward TTTCTACATCAACCTTACTGCACC Reverse CACCGCAGAAGAAGATTACATAGG
SSR size	170, 3B locus
Amplification Conditions	61C anneal
Sequence	PSP3144
Source Species	Triticum aestivum