GrainGenes Marker Report: Xwmc0397.1-6B
Locus
Xwmc0397.1-6B
Type
Microsatellite
Map
P91193D1/P92201D5-6B 149.8 Wheat_Yr78_2017-6B 50.9
[ Show Nearby Loci ]
Map Data
Wheat, P91193D1 x P92201D5 Wheat_Yr78_2017
Probe
WMC397
Possible Orthologs
Xwmc397-6B Xwmc397b wmc397
Probe for Locus
WMC397
Locus
Xwmc397-6B Xwmc0397.1-6B Xwmc397b wmc397
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 10h12 Donor: Jacquemin, Gembloux Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 162 bases located on 3A, 364 bases located on 4B 5' end not located, trimmed to Rsa1 at 3' end. (CA)9 69 to 86, (GA)14 93 to 120.
Type
PCR SSR
PCR primers
Forward AgTcgTgcAccTccATTTTg at 46 length 20 bases, Tm 63
Reverse cATTggAcATcggAgAccTg at 186 length 20 bases, Tm 63
SSR size
160 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide.
Sequence
WMC397
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc0397.1-6B
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