GrainGenes Marker Report: Xwmc0532-3A
Locus
Xwmc0532-3A
Type
Microsatellite
Map
P91193D1/P92201D5-3A 18.1
[ Show Nearby Loci ]
Map Data
Wheat, P91193D1 x P92201D5
Probe
WMC532
Possible Orthologs
Xwmc532 Xwmc532-3A wmc532 wmc532-3A
Probe for Locus
WMC532
Locus
Xwmc532-3A Xwmc532 Xwmc0532-3A wmc532 wmc532-3A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 26c1 Donor: Madre, Secobra Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 180 bases located on 3A Trimmed to insert at both ends. Bases 84 to 105 (complement) show homology to 9a6 bases 751 to 772 (22 in longest run, maxScore = 61). Forward primer has at least 5 mismatches to 9a6, reverse has no real match to 9a6. (GA)5 72 to 81, (GA)11 84 to 105.
Type
PCR SSR
PCR primers
Forward gATAcATcAAgATcgTgccAAA at 40 length 20 bases, Tm 62
Reverse gggAgAAATcATTAAcgAAggg at 194 length 20 bases, Tm 62
SSR size
176 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (160-180 bases).
Sequence
WMC532
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc0532-3A
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