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GrainGenes Marker Report: Xwmc104-6B

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Locus	Xwmc104-6B
Type	Microsatellite
Chromosome	6B
Chromosome Arm	6BS
Map	Ta-SSR-2004-6B 17 Ta-Synthetic/Opata-GPW-6B 36.9 Ta-Synthetic/Opata-SSR2-6B 42.2
	[ Show Nearby Loci ]
Map Data	Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR 2
Probe	WMC104
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc104 Xwmc104-1A wmc104 wmc104-6B
Probe for Locus	WMC104
Locus	Xwmc104-1A Xwmc104-6B Xwmc104 wmc104 wmc104-6B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7d5 Donor: Somers, Agriculture Canada Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 132 bases located on 6B, 143 bases located on 6B, 95 bases located on 6B Trimmed to insert (Rsa1 at 3'). (GT)31 44 to 105. (2 loci both polymorphic?). Lower locus is 7D - linked to preharvest sprouting in SPR8198 x HD2329 ( Roy et al Theor Appl Gene 99 336-340 (1999))
Type	PCR SSR
PCR primers	Forward TcTcccTcATTAgAgTTgTccA at 21 length 22 bases, Tm 61 Reverse ATgcAAgTTTAgAgcAAcAccA at 139 length 22 bases, Tm 61
SSR size	140 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC104
Source Species	Triticum aestivum