Query (optional)   in Class  

GrainGenes Marker Report: Xwmc105-6B

[Submit comment/correction]

Locus
Xwmc105-6B
Type
Microsatellite
Chromosome
6B
Chromosome Arm
6BS
Map
Kofa/UC1113-6B60.4
Ta-SSR-2004-6B40.2
Ta-Synthetic/Opata-GPW-6B48.2
Ta-Synthetic/Opata-SSR2-6B57.1
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113
Wheat, Consensus SSR, 2004
Wheat, Synthetic x Opata, GPW
Wheat, Synthetic x Opata, SSR 2
Probe
WMC105
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc105
Xwmc105y
wmc105
wmc105-6B



Probe for Locus
WMC105
Locus
Xwmc105-6B
Xwmc105
Xwmc105y
wmc105
wmc105-6B
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 7f5
Donor: Somers, Agriculture Canada
Trimmed to insert (Rsa1 at 5' end). (GT)14 77 to 104. (2 loci - both polymorphic?)
Type
PCR
SSR
PCR primers
Forward AATgTcATgcgTgTAgTAgccA at 6 length 22 bases, Tm 62
Reverse AAgcgcAcTTAAcAgAAgAggg at 176 length 22 bases, Tm 64
SSR size
192 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC105
Source Species
Triticum aestivum