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GrainGenes Marker Report: Xwmc105y

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Locus	Xwmc105y
Type	Microsatellite
Chromosome	6B
Map	Wheat-Composite2004-6B 114
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Map Data	Wheat, Composite, 2004
Probe	WMC105
Possible Orthologs	Xwmc105 Xwmc105-6B wmc105 wmc105-6B
Probe for Locus	WMC105
Locus	Xwmc105-6B Xwmc105 Xwmc105y wmc105 wmc105-6B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7f5 Donor: Somers, Agriculture Canada Trimmed to insert (Rsa1 at 5' end). (GT)14 77 to 104. (2 loci - both polymorphic?)
Type	PCR SSR
PCR primers	Forward AATgTcATgcgTgTAgTAgccA at 6 length 22 bases, Tm 62 Reverse AAgcgcAcTTAAcAgAAgAggg at 176 length 22 bases, Tm 64
SSR size	192 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC105
Source Species	Triticum aestivum