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GrainGenes Marker Report: Xwmc109-2A

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Locus
Xwmc109-2A
Type
Microsatellite
Chromosome
2A
Map
Ta-SSR-2004-2A70.7
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC109
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc109d
wmc109



Probe for Locus
WMC109
Locus
Xwmc109d
Xwmc109-2A
wmc109
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 8a8
Donor: Flore, Verneuil
AGR164. Trimmed to insert at both ends. (CA)14 54 to 81. Bands or right size are a little weak to be sure of polymorphism.
Type
PCR
SSR
PCR primers
Forward AATTcgggAAgAgTcTcAgggg at 6 length 22 bases, Tm 67
Reverse TTcgAAgggcTcAAgggATAcg at 173 length 22 bases, Tm 68
SSR size
189 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC109
Source Species
Triticum aestivum