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GrainGenes Marker Report: Xwmc109d

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Locus	Xwmc109d
Type	Microsatellite
Chromosome	2A
Map	Wheat-Composite2004-2A 78
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Map Data	Wheat, Composite, 2004
Probe	WMC109
Possible Orthologs	Xwmc109-2A wmc109
Probe for Locus	WMC109
Locus	Xwmc109d Xwmc109-2A wmc109
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8a8 Donor: Flore, Verneuil AGR164. Trimmed to insert at both ends. (CA)14 54 to 81. Bands or right size are a little weak to be sure of polymorphism.
Type	PCR SSR
PCR primers	Forward AATTcgggAAgAgTcTcAgggg at 6 length 22 bases, Tm 67 Reverse TTcgAAgggcTcAAgggATAcg at 173 length 22 bases, Tm 68
SSR size	189 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC109
Source Species	Triticum aestivum