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GrainGenes Marker Report: Xwmc11

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Locus
Xwmc11
Type
Microsatellite
Chromosome
3A
Map
Ta-NxW-3B32.6
Wheat, UC1110 x PI610750 3A27
Wheat-Composite2004-3A23
Wheat_Yr76_2016-3A10.5
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Wheat, Nanda2419 x Wangshuibai
Wheat, UC1110 x PI610750
Wheat_Yr76_2016
Probe
WMC11
Reference
ReferenceLowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs
Xwmc11-1A
[ Show all 19 ]



Probe for Locus
WMC11
Locus
Xwmc11a-3A
[ Show all 21 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 1H4
Donor: Nickerson
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 184 bases located on 3A, 240 bases located on 3D
AGR32. CT microstellite. Expected fragment 177 bases. Products obtained after PCR 61C anneal. (2loci?).
Type
PCR
SSR
PCR primers
Forward TTgTgATccTggTTgTgTTgTgA at 95 length 23 bases, Tm 65
Reverse cAcccAgccgTTATATATgTTgA at 249 length 23 bases, Tm 62
SSR size
177 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC11
Source Species
Triticum aestivum