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GrainGenes Marker Report: Xwmc11-3A

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Locus
Xwmc11-3A
Type
Microsatellite
Chromosome
3A
Map
Louise/Penawawa-3A0.7
T.b.xT.m.-3A34.3
Ta-SSR-2004-3A0
Ta-Synthetic/Opata-GPW-3A-3.5
Wheat_Yr76_2016-3A10.5
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Map Data
T. boeoticum x monococcum
Wheat, Consensus SSR, 2004
Wheat, Louise x Penawawa
Wheat, Synthetic x Opata, GPW
Wheat_Yr76_2016
Probe
WMC11
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc11
[ Show all 19 ]



Probe for Locus
WMC11
Locus
Xwmc11a-3A
[ Show all 21 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 1H4
Donor: Nickerson
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 184 bases located on 3A, 240 bases located on 3D
AGR32. CT microstellite. Expected fragment 177 bases. Products obtained after PCR 61C anneal. (2loci?).
Type
PCR
SSR
PCR primers
Forward TTgTgATccTggTTgTgTTgTgA at 95 length 23 bases, Tm 65
Reverse cAcccAgccgTTATATATgTTgA at 249 length 23 bases, Tm 62
SSR size
177 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC11
Source Species
Triticum aestivum