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GrainGenes Marker Report: Xwmc111-2D

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Locus	Xwmc111-2D
Type	Microsatellite
Chromosome	2D
Map	CS/SQ1-2D 10.2 Ta-SSR-2004-2D 11 Ta-Synthetic/Opata-GPW-2D 51.4
	[ Show Nearby Loci ]
Map Data	Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW
Probe	WMC111
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc111
Probe for Locus	WMC111
Locus	Xwmc111-2D Xwmc111
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8e8 Donor: Flore, Verneuil Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 239 bases located on 2D AGR168. Trimmed to Rsa1 at both ends. (CA)9 152 to 169, (CA)15 176 to 205.
Type	PCR SSR
PCR primers	Forward ATTgATgTgTAcgATgTgccTg at 56 length 22 bases, Tm 63 Reverse cATgTcAATgTcATgATgAAgc at 276 length 22 bases, Tm 61
SSR size	243 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC111
Source Species	Triticum aestivum