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GrainGenes Marker Report: Xwmc113c

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Locus	Xwmc113c
Type	Microsatellite
Chromosome	1B
Map	Wheat-Composite2004-1B 113
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Map Data	Wheat, Composite, 2004
Probe	WMC113
Possible Orthologs	Xwmc113-1B Xwmc113-6D Xwmc113x
Probe for Locus	WMC113
Locus	Xwmc113-1B Xwmc113-6D Xwmc113c Xwmc113x
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8f9 Donor: Flore, Verneuil AGR177. Trimmed to insert 5' and to Rsa1 at 3'. (GT)18 36 to 71. Redesign primers if possible.
Type	PCR SSR
PCR primers	Forward cAAgTTgAccATcgAcTcATAA at 8 length 22 bases, Tm 59 Reverse AAcTggAAgccAcgAcAAcATA at 73 length 22 bases, Tm 63
SSR size	87 bases expected, Chinese Spring
Amplification Conditions	51C anneal, smear;
Sequence	WMC113
Source Species	Triticum aestivum