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GrainGenes Marker Report: Xwmc115

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Locus	Xwmc115
Type	Microsatellite
Map	Ta-NxW-5D 82.1
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai
Probe	WMC115
Probe for Locus	WMC115
Locus	Xwmc115
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8b11 Donor: Flore, Verneuil AGR189. Trimmed to insert. (GT)13 72 to 97. Try 70C anneal.
Type	PCR SSR
PCR primers	Forward ggAccccAgTTgcATgTggAcc at 42 length 22 bases, Tm 72 Reverse TgcAATTgggggccTcgAgAAA at 97 length 22 bases, Tm 73
SSR size	77 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear; 51C anneal, smear;
Sequence	WMC115
Source Species	Triticum aestivum