GrainGenes Marker Report: Xwmc11a-3A
Locus
Xwmc11a-3A
Type
Microsatellite
Map
CS/SQ1-3A 8.8
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1
Probe
WMC11
Possible Orthologs
Xwmc11
[ Show all 19 ]
Probe for Locus
WMC11
Locus
Xwmc11a-3A
[ Show all 21 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 1H4 Donor: Nickerson Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 184 bases located on 3A, 240 bases located on 3D AGR32. CT microstellite. Expected fragment 177 bases. Products obtained after PCR 61C anneal. (2loci?).
Type
PCR SSR
PCR primers
Forward TTgTgATccTggTTgTgTTgTgA at 95 length 23 bases, Tm 65
Reverse cAcccAgccgTTATATATgTTgA at 249 length 23 bases, Tm 62
SSR size
177 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC11
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc11a-3A
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