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GrainGenes Marker Report: Xwmc121-7D

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Locus
Xwmc121-7D
Type
Microsatellite
Chromosome
7D
Map
Ta-SSR-2004-7D85.9
Ta-Synthetic/Opata-GPW-7D128.1
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Wheat, Synthetic x Opata, GPW
Probe
WMC121
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc121
wmc121



Probe for Locus
WMC121
Locus
Xwmc121-7D
Xwmc121
wmc121
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 8e12
Donor: Flore, Verneuil
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 309 bases located on 7D
AGR200. Trimmed to insert at 5' end and to Rsa1 site at 3' end. (GA)12 207 to 230. Maybe polymorphic.
Type
PCR
SSR
PCR primers
Forward ggcTgTggTcTcccgATcATTc at 26 length 22 bases, Tm 69
Reverse AcTggAcTTgAggAggcTggcA at 312 length 22 bases, Tm 69
SSR size
308 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product;
Sequence
WMC121
Source Species
Triticum aestivum