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GrainGenes Marker Report: Xwmc125-4B

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Locus	Xwmc125-4B
Type	Microsatellite
Chromosome	4B
Chromosome Arm	4BL
Map	Louise/Penawawa-4B 0 P91193D1/P92201D5-4B 106.7 Ta-SSR-2004-4B 59.4 Triticum-WxM-FHB-QTL-4B 75.3
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Map Data	Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa Wheat, P91193D1 x P92201D5 Wheat, WxM, FHB QTL
Probe	WMC125
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Somers DJ et al. (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat Genome 46:555-564.
Possible Orthologs	Xwmc125 wmc125
Probe for Locus	WMC125
Locus	Xwmc125-4B Xwmc125 wmc125
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7g8 Donor: Blake, Montana Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 256 bases located on 4B Microsatellite : [TA]5 TGC [GT]11 GA [GT]20 Tom Primer 1 : AGTTG ACTTC ATATG TTAGG (35%) Tom Primer 2 : TTATC GAAGG ACTAC GATAG (40%) EXPECTED PCR PRODUCT: 181 nts.(GT)11 213 to 234, (GT)20 237 to 276,
Type	PCR SSR
PCR primers	Forward ATAccAccATgcATgTggAAgT at 138 length 22 bases, Tm 63 Reverse AccgcTTgTcATTTccTTcTgT at 367 length 22 bases, Tm 64
SSR size	251 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (alleles 234-250).
Sequence	WMC125
Source Species	Triticum aestivum