GrainGenes Marker Report: Xwmc126-5A
Locus
Xwmc126-5A
Type
Microsatellite
Chromosome
5A
Chromosome Arm
5AL
Map
Triticum-WxM-FHB-QTL-5A 144.4
[ Show Nearby Loci ]
Map Data
Wheat, WxM, FHB QTL
Probe
WMC126
Reference
Somers DJ et al. (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat Genome 46:555-564.
Probe for Locus
WMC126
Locus
Xwmc126-5A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 7b9 Donor: Blake, Montana 7B9 Microsatellite : [GT]~60 [AC]~30 COMBINATION. Size of microsatellite only approximate. (CA)28 257 to 312, (GT)60 136 to 255.
Type
PCR SSR
PCR primers
Forward TTgTcAcAAAAgggTcgTAAAT at 48 length 22 bases, Tm 60
Reverse ATcTcgAgATgTcATcgAAAAT at 362 length 59 bases, Tm 59
SSR size
336 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic on acrylamide (nulls for 188 base allele).
Sequence
WMC126
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc126-5A
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