GrainGenes Marker Report: Xwmc128-1B
Locus
Xwmc128-1B
Type
Microsatellite
Chromosome
1B
Map
Ta-SSR-2004-1B 29.8
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC128
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
wmc128 wmc128-1B
Probe for Locus
WMC128
Locus
Xwmc128-1B wmc128 wmc128-1B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 7c10 Donor: Blake, Montana Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 207 bases located on 1B Microsatellite : [GT]16 [GA]10. Tom Primer 1 : CGAAT CGGAC AGCTA CTGCT (55%). Tom Primer 2 : CACCC TTGTG TGTCC ACGTT (55%). EXPECTED PCR PRODUCT: 134 nts. (GA)10 195 to 214, (GT)16 163 to 194. Bases 163-291 (ie. 3' side of microsatellite) homologous to part of 2g10 (reverse primer in this domain).
Type
PCR SSR
PCR primers
Forward cggAcAgcTAcTgcTcTccTTA at 73 length 22 bases, Tm 63
Reverse cTgTTgcTTgcTcTgcAcccTT at 250 length 22 bases, Tm 68
SSR size
199 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product (202 bases).
Sequence
WMC128
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc128-1B
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