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GrainGenes Marker Report: Xwmc134-1B

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Locus	Xwmc134-1B
Type	Microsatellite
Chromosome	1B
Map	Ta-SSR-2004-1B 47.3
	[ Show Nearby Loci ]
Map Data	Wheat, Consensus SSR, 2004
Probe	WMC134
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc134 wmc134 wmc134-1B
Probe for Locus	WMC134
Locus	Xwmc134 Xwmc134-1B wmc134 wmc134-1B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7c12 Donor: Blake, Montana Microsatellite : [TG]18. Tom Primer 1 : CCAAG CTGTC TGACT GCCAT (55%). Tom Primer 2 : TATAG ACCTC TGGCT CACGG (55%). TOM's EXPECTED PCR PRODUCT: 135 nts(GT)17 161 to 194.
Type	PCR SSR
PCR primers	Forward ccAAgcTgTcTgAcTgccATAg at 86 length 22 bases, Tm 64 Reverse AgTATAgAccTcTggcTcAcgg at 201 length 22 bases, Tm 61
SSR size	137 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (152-174 bases).
Sequence	WMC134
Source Species	Triticum aestivum