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GrainGenes Marker Report: Xwmc141

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Locus	Xwmc141
Type	Microsatellite
Map	Ta-NxW-4B 108
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai
Probe	WMC141
Probe for Locus	WMC141
Locus	Xwmc141
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5a9 Donor: Henry, Lismore Trimmed to insert at 3' end (CA)15 66 to 95. Complex.
Type	PCR SSR
PCR primers	Forward TgcAAggggTTgAgTTcTTAgg at 40 length 22 bases, Tm 64 Reverse TTgATAgAgcAAgAcgAAgggc at 117 length 22 bases, Tm 65
SSR size	99 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC141
Source Species	Triticum aestivum