GrainGenes Marker Report: Xwmc144-2D
Locus
Xwmc144-2D
Type
Microsatellite
Chromosome
2D
Map
Conan/Reeder-2D 67.262 Louise/Penawawa-2D 38.6 Ta-SSR-2004-2D 67.1 Wheat-2008-Coda-Brundage-2D 35
[ Show Nearby Loci ]
Map Data
Wheat 2008, Coda x Brundage, C gene Wheat, Conan x Reeder Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa
Probe
WMC144
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
wmc144
Probe for Locus
WMC144
Locus
Xwmc144-2D wmc144
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 5g10 Donor: Henry, Lismore Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 144 bases located on 2D (CA)14 69 to 96. Bases 70-247 (complement) ie 3' side of microsatellite match 1b4, 5f2.
Type
PCR SSR
PCR primers
Forward ggAcAccAATccAAcATgAAcA at 7 length 22 bases, Tm 65
Reverse AAggATAgTTgggTggTgcTgA at 128 length 22 bases, Tm 65
SSR size
143 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product;
Sequence
WMC144
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc144-2D
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