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GrainGenes Marker Report: Xwmc147-1D

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Locus	Xwmc147-1D
Type	Microsatellite
Chromosome	1D
Chromosome Arm	1DS
Map	Ta-SSR-2004-1D 16.4 Ta-Synthetic/Opata-GPW-1D 17.5 Ta-Synthetic/Opata-SSR2-1D 18.5
	[ Show Nearby Loci ]
Map Data	Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR 2
Probe	WMC147
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc147 Xwmc147-3A wmc147 WMC147-1D
Probe for Locus	WMC147
Locus	Xwmc147-1D Xwmc147 Xwmc147-3A wmc147 WMC147-1D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11b1 Donor: Rogers, Monsanto Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 112 bases located on 1D, 119 bases located on 1D, 130 bases located on 1D AGR204.2.SEQ. Trimmed to Rsa1 at 5', and insert at 3'.(CA)15 99 to 128. (3 loci).
Type	PCR SSR
PCR primers	Forward AgAAcgAAAgAAgcgcgcTgAg at 18 length 22 bases, Tm 69 Reverse ATgTgTTTcTTATccTgcgggc at 148 length 22 bases, Tm 66
SSR size	152 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC147
Source Species	Triticum aestivum