GrainGenes Marker Report: Xwmc149.2
Locus
Xwmc149.2
Type
Microsatellite
Map
Ta-NxW-5B 35.5
[ Show Nearby Loci ]
Map Data
Wheat, Nanda2419 x Wangshuibai
Probe
WMC149
Possible Orthologs
Xwmc149
[ Show all 16 ]
Probe for Locus
WMC149
Locus
Xwmc149a-5B
[ Show all 17 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 11h1 Donor: Rogers, Monsanto Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 180 bases located on 5B, 185 bases located on 1B, 230 bases located on 2B AGR204.8.SEQ. Trimmed to Rsa1 at both ends. (CT)8 50 to 65, (CT)24 68 to 115. (3-4 loci, 2-3 polymorphic).
Type
PCR SSR
PCR primers
Forward AcAgAcTTggTTggTgccgAgc at 6 length 22 bases, Tm 70
Reverse ATgggcgggggTgTAgAgTTTg at 214 length 22 bases, Tm 70
SSR size
230 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC149
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc149.2
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