GrainGenes Marker Report: Xwmc15-4A
Locus
Xwmc15-4A
Type
Microsatellite
Chromosome
4A
Map
Ta-SSR-2004-4A 9.1
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC15
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
wmc15
Probe for Locus
WMC15
Locus
Xwmc15-4A wmc15
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 1H5 Donor: Nickerson AGR40. (CT) (CA) compound microsatellite. expected fragment=295. (2 loci?). Bases 223 to 245 show homology to 21e1 bases 121 to 143 (complement) (23 in longest run, maxScore = 25).
Type
PCR SSR
PCR primers
Forward AgTccgATTcggAcTccTcAAg at 164 length 22 bases, Tm 66
Reverse ggAcTAAccgAgggTAgTTcAg at 437 length 22 bases, Tm 61
SSR size
295 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC15
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc15-4A
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