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GrainGenes Marker Report: Xwmc150c

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Locus	Xwmc150c
Type	Microsatellite
Chromosome	5A
Map	Wheat-Composite2004-5A 29
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC150
Possible Orthologs	Xwmc150 [ Show all 14 ]
Probe for Locus	WMC150
Locus	Xwmc150.1-2A [ Show all 15 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11h2 Donor: Rogers, Monsanto AGR204.16.SEQ No overlap between forward and reverse sequences. Trimmed to insert at both ends. (GT)21 472 to 513. (5 loci, 3 polymorphic?). Complment of forward sequence matches 2c8, 6a6, 6b6, 7a2. Bases 90 to 110 show homology to 12a9 bases 461 to 481 (complement) (21 in longest run, maxScore = 113). Bases 225 to 285 show homology to 22h9 bases 71 to 131 (61 in longest run, maxScore = 92). Bases 123 to 287 show homology to 24d1 bases 7 to 171 (complement) (165 in longest run, maxScore = 171), duplicate, 11h2 has priority.
Type	PCR SSR
PCR primers	Forward cATTgATTgAAcAgTTgAAgAA at 358 length 22 bases, Tm 57 Reverse cTcAAAgcAAcAgAAAAgTAAA at 501 length 22 bases, Tm 56
SSR size	165 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC150
Source Species	Triticum aestivum