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GrainGenes Marker Report: Xwmc153

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Locus	Xwmc153
Type	Microsatellite
Chromosome	1D 3A
Map	Svevo/Ciccio-3A 121 Ta-NxW-3A 150.4 Wheat, UC1110 x PI610750 3A 141.7 Wheat-Composite2004-1D 3 Wheat-Composite2004-3A 98
	[ Show Nearby Loci ]
Breakpoint Interval	C-3AL3-0.42
Map Data	Durum wheat, Svevo x Ciccio Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMC153
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc153-1D Xwmc153-3A wmc153 wmc153-3A
Probe for Locus	WMC153
Locus	Xwmc153-1D Xwmc153 Xwmc153-3A wmc153 wmc153-3A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11f3 Donor: Rogers, Monsanto Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 172-184 bases located on 3A AGR204.22.SEQ. Trimmed to insert at 5' and 3' end. (GT)20 54 to 93, (GT)18 95 to 130, (GT)11 132 to 153. 3-4 loci. one smeary polymorphism, 1 small polymorphism.
Type	PCR SSR
PCR primers	Forward ATgAggAcTcgAAgcTTggc at 4 length 20 bases, Tm 64 Reverse cTgAgcTTTTgcgcgTTgAc at 161 length 20 bases, Tm 66
SSR size	177 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC153
Source Species	Triticum aestivum