GrainGenes Marker Report: Xwmc153-1D
Locus
Xwmc153-1D
Type
Microsatellite
Map
Ta-Synthetic/Opata-GPW-1D 221.3
[ Show Nearby Loci ]
Map Data
Wheat, Synthetic x Opata, GPW
Probe
WMC153
Possible Orthologs
Xwmc153 Xwmc153-3A wmc153 wmc153-3A
Probe for Locus
WMC153
Locus
Xwmc153-1D Xwmc153 Xwmc153-3A wmc153 wmc153-3A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 11f3 Donor: Rogers, Monsanto Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 172-184 bases located on 3A AGR204.22.SEQ. Trimmed to insert at 5' and 3' end. (GT)20 54 to 93, (GT)18 95 to 130, (GT)11 132 to 153. 3-4 loci. one smeary polymorphism, 1 small polymorphism.
Type
PCR SSR
PCR primers
Forward ATgAggAcTcgAAgcTTggc at 4 length 20 bases, Tm 64
Reverse cTgAgcTTTTgcgcgTTgAc at 161 length 20 bases, Tm 66
SSR size
177 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC153
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc153-1D
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