GrainGenes Marker Report: Xwmc153-3A
Locus
Xwmc153-3A
Type
Microsatellite
Chromosome
3A
Chromosome Arm
3AL
Map
T.b.xT.m.-3A 198.7 Ta-SSR-2004-3A 87.4 Ta-Synthetic/Opata-SSR2-3A 115.1 Wheat_Brittle_culm_2012-3A 0
[ Show Nearby Loci ]
Map Data
Durum wheat, Svevo x Ciccio T. boeoticum x monococcum Wheat, 2012, Brittle culm Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, SSR 2
Probe
WMC153
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc153 Xwmc153-1D wmc153 wmc153-3A
Probe for Locus
WMC153
Locus
Xwmc153-1D Xwmc153 Xwmc153-3A wmc153 wmc153-3A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 11f3 Donor: Rogers, Monsanto Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 172-184 bases located on 3A AGR204.22.SEQ. Trimmed to insert at 5' and 3' end. (GT)20 54 to 93, (GT)18 95 to 130, (GT)11 132 to 153. 3-4 loci. one smeary polymorphism, 1 small polymorphism.
Type
PCR SSR
PCR primers
Forward ATgAggAcTcgAAgcTTggc at 4 length 20 bases, Tm 64
Reverse cTgAgcTTTTgcgcgTTgAc at 161 length 20 bases, Tm 66
SSR size
177 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC153
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc153-3A
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
|