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GrainGenes Marker Report: Xwmc154

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Locus	Xwmc154
Type	Microsatellite
Chromosome	2B
Map	Ta-NxW-2B 49.6 Wheat, UC1110 x PI610750 2B 35.1 Wheat-Composite2004-2B 35
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMC154
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc154-2B wmc154 wmc154-2B
Probe for Locus	WMC154
Locus	Xwmc154-2B Xwmc154 wmc154 wmc154-2B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11f4 Donor: Rogers, Monsanto Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 149 bases located on 2B AGR204.30.SEQ. Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)34 118 to 185. (2 loci - 1 clear polymorphism). Bases 27 to 116 show homology to 25h1 bases 1 to 90 (90 in longest run, maxScore = 81). Duplicate with 25h1, 11f4 has priority.
Type	PCR SSR
PCR primers	Forward ATgcTcgTcAgTgTcATgTTTg at 74 length 22 bases, Tm 63 Reverse AAAcggAAccTAccTcAcTcTT at 199 length 22 bases, Tm 60
SSR size	147 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC154
Source Species	Triticum aestivum