GrainGenes Marker Report: Xwmc154-2B
Locus
Xwmc154-2B
Type
Microsatellite
Chromosome
2B
Chromosome Arm
2BS
Map
Kofa/UC1113-2B 0 Ta-SSR-2004-2B 28.5 Ta-Synthetic/Opata-GPW-2B 41.5 Ta-Synthetic/Opata-SSR2-2B 84.7
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113 Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR 2
Probe
WMC154
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc154 wmc154 wmc154-2B
Probe for Locus
WMC154
Locus
Xwmc154-2B Xwmc154 wmc154 wmc154-2B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 11f4 Donor: Rogers, Monsanto Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 149 bases located on 2B AGR204.30.SEQ. Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)34 118 to 185. (2 loci - 1 clear polymorphism). Bases 27 to 116 show homology to 25h1 bases 1 to 90 (90 in longest run, maxScore = 81). Duplicate with 25h1, 11f4 has priority.
Type
PCR SSR
PCR primers
Forward ATgcTcgTcAgTgTcATgTTTg at 74 length 22 bases, Tm 63
Reverse AAAcggAAccTAccTcAcTcTT at 199 length 22 bases, Tm 60
SSR size
147 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC154
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc154-2B
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