GrainGenes Marker Report: Xwmc156.2
Locus
Xwmc156.2
Type
Microsatellite
Chromosome
1B
Map
Wheat-Composite2004-1B 52
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMC156
Possible Orthologs
Xwmc156-1B wmc156-1B wmc156
Probe for Locus
WMC156
Locus
Xwmc156-1B Xwmc156.2 wmc156-1B wmc156
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 11h4 Donor: Rogers, Monsanto Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 237 bases located on 1B AGR204.32.SEQ. Trimmed to insert at both ends. Multiple inserts in this clone joined 230-237 by linkers. (CA)10 30 to 49, (GCC)6 317 to 334. Primers shown here are for first insert. Second insert has complex repeating structure - use as minisatellite probe? Complex band pattern.
Type
PCR SSR
PCR primers
Forward gccTcTAgggAgAAAAcTAAcA at 6 length 22 bases, Tm 58
Reverse TcAAgATcATATccTccccAAc at 195 length 22 bases, Tm 62
SSR size
211 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product;
Sequence
WMC156
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc156.2
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