Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: Xwmc157-7D

[Submit comment/correction]

Locus	Xwmc157-7D
Type	Microsatellite
Chromosome	7D
Chromosome Arm	7DL
Map	CS/SQ1-7D 213.4 Ta-Synthetic/Opata-GPW-7D 214 Ta-Synthetic/Opata-SSR2-7D 255.4
	[ Show Nearby Loci ]
Map Data	Wheat, Chinese Spring x SQ1 Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR 2
Probe	WMC157
Possible Orthologs	Xwmc157 wmc157
Probe for Locus	WMC157
Locus	Xwmc157-7D [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11c5 Donor: Rogers, Monsanto AGR204.35.SEQ. Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)29 212 to 269. Redesign primers? Bases 115-229 (ie 5' side of microsatellite) match part of 5g9. Bases 189 to 263 show homology to 24c1 bases 82 to 156 (75 in longest run, maxScore = 82), duplicate, 11c5 has precedence.
Type	PCR SSR
PCR primers	Forward cTTgATccAAgTggTTcTTTcc at 183 length 22 bases, Tm 62 Reverse TccAAATgTTTgcgAAAccTgA at 270 length 22 bases, Tm 66
SSR size	109 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear; 51C anneal, product;
Sequence	WMC157
Source Species	Triticum aestivum