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GrainGenes Marker Report: Xwmc158-7A

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Locus
Xwmc158-7A
Type
Microsatellite
Chromosome
7A
Map
CS/SQ1-7A18
Ta-SSR-2004-7A5.4
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1
Wheat, Consensus SSR, 2004
Probe
WMC158
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc158



Probe for Locus
WMC158
Locus
Xwmc158-7A
Xwmc158
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 11b6
Donor: Rogers, Monsanto
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 369 bases located on 7A
AGR204.42.SEQ. Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)17 287 to 320, (CT)24 119 to 166, (CT)9 197 to 214, (CT)9 241 to 258.
Type
PCR
SSR
PCR primers
Forward AAcTggcATcATgTTTTgTAgg at 27 length 22 bases, Tm 61
Reverse AATgTAgTcAAAAgAggTggTg at 382 length 22 bases, Tm 58
SSR size
377 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product;
Sequence
WMC158
Source Species
Triticum aestivum