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GrainGenes Marker Report: Xwmc16-4B

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Locus	Xwmc16-4B
Type	Microsatellite
Chromosome	4B
Map	Ta-SSR-2004-4B 24.5
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Map Data	Wheat, Consensus SSR, 2004
Probe	WMC16
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	wmc16 wmc16-2B wmc16-4B
Probe for Locus	WMC16
Locus	Xwmc16-4B wmc16 wmc16-2B wmc16-4B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1A6 Donor: Nickerson Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 165 bases located on 4B AGR41. CT microsatellite. Expected fragment size 109. Small smeary products obtained on agarose after PCR 61C anneal.
Type	PCR SSR
PCR primers	Forward AccgccTgcATTcTcATcTAcA at 26 length 22 bases, Tm 66 Reverse gTggcgccATggTAgAgATTTg at 113 length 22 bases, Tm 68
SSR size	109 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear;
Sequence	WMC16
Source Species	Triticum aestivum