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GrainGenes Marker Report: Xwmc161a

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Locus	Xwmc161a
Type	Microsatellite
Chromosome	4A
Map	Wheat-Composite2004-4A 105
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC161
Possible Orthologs	Xwmc161 [ Show all 17 ]
Probe for Locus	WMC161
Locus	Xwmc161-4A [ Show all 18 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11f6 Donor: Rogers, Monsanto AGR204.46.SEQ No overlap between forward and reverse sequences. Insert appears to contain multiple inserts (2) joined by linker sequence at 334-341. Trimmed to insert at both ends. (GT)25 245 to 294. Reverse primer begins GT (first part of Rsa1 site in the genome - does not appear in sequence above as replaced by linker). Bases 1 to 279 show homology to 12h6.1 bases 1 to 279 (279 in longest run, maxScore = 211). Bases 362 to 639 show homology to 12h6.2 bases 50 to 327 (278 in longest run, maxScore = 239).
Type	PCR SSR
PCR primers	Forward AccTTcTTTgggATggAAgTAA at 172 length 22 bases, Tm 61 Reverse gTAcTgAAccAcTTgTAAcgcA at 312 length 22 bases, Tm 60
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC161
Source Species	Triticum aestivum