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GrainGenes Marker Report: Xwmc163

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Locus	Xwmc163
Type	Microsatellite
Chromosome	6A
Map	Wheat-Composite2004-6A 102
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Map Data	Wheat, Composite, 2004
Probe	WMC163
Possible Orthologs	Xwmc163-6A
Probe for Locus	WMC163
Locus	Xwmc163-6A Xwmc163
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9A1 Donor: Bernard, Clermont-Ferrand Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)8 327 to 342. Duplicate of 13H1.
Type	PCR SSR
PCR primers	Forward TTAcAcccATcAgggTggTcTT at 65 length 22 bases, Tm 64 Reverse gTccgTcTATccATAcgAcAAA at 349 length 22 bases, Tm 61
SSR size	306 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC163
Source Species	Triticum aestivum