GrainGenes Marker Report: Xwmc166-7B
Locus
Xwmc166-7B
Type
Microsatellite
Chromosome
7B
Chromosome Arm
7BL
Map
Ta-Courtot/CS-GPW-7B 143.7 Ta-SSR-2004-7B 141.8 Ta-Synthetic/Opata-GPW-7B 193.8 Ta-Synthetic/Opata-SSR2-7B 194.5
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR 2
Probe
WMC166
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc166
[ Show all 7 ]
Probe for Locus
WMC166
Locus
Xwmc166-7B
[ Show all 8 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 9g1 Donor: Bernard, Clermont-Ferrand Trimmed to Rsa1 at 5' end and to insert at 3' end. (GA)8 347 to 362, (GT)8 297 to 312, (GT)8 327 to 342. (Near) Duplicate of first insert in 3E8. Bases 60 to 310 show homology to 14f6 bases 1 to 251 (251 in longest run, maxScore = 183).
Type
PCR SSR
PCR primers
Forward ATAAAgcTgTcTcTTTAgTTcg at 81 length 22 bases, Tm 54
Reverse gTTTTAAcAcATATgcATAccT at 364 length 22 bases, Tm 53
SSR size
305 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC166
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc166-7B
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