GrainGenes Marker Report: Xwmc167-2B
Locus
Xwmc167-2B
Type
Microsatellite
Map
Kofa/UC1113-2B 96.1
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113
Probe
WMC167
Possible Orthologs
Xwmc167 Xwmc167-2D wmc167-2B wmc167 wmc167-2D
Probe for Locus
WMC167
Locus
Xwmc167-2D
[ Show all 6 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 9b2 Donor: Bernard, Clermont-Ferrand Trimmed to insert at both ends. Multiples inserts (2) in this clone separated 380 -397 by linkers. (CA)22 87 to 130, (CA)8 535 to 550, (CA)8 553 to 568. Primers are for first insert in this clone.
Type
PCR SSR
PCR primers
Forward AgTggTAATgAggTgAAAgAAg at 7 length 22 bases, Tm 57
Reverse TcggTcgTATATgcATgTAAAg at 170 length 22 bases, Tm 59
SSR size
185 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC167
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc167-2B
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