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GrainGenes Marker Report: Xwmc167-2D

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Locus
Xwmc167-2D
Type
Microsatellite
Chromosome
2D
Chromosome Arm
2DL
Map
Conan/Reeder-2D146.261
Ta-Courtot/CS-GPW-2D238.4
Ta-SSR-2004-2D97.1
Ta-Synthetic/Opata-GPW-2D181.6
Ta-Synthetic/Opata-SSR2-2D204.1
[ Show Nearby Loci ]
Map Data
Wheat, Conan x Reeder
Wheat, Consensus SSR, 2004
Wheat, Courtot x Chinese Spring, GPW
Wheat, Synthetic x Opata, GPW
Wheat, Synthetic x Opata, SSR 2
Probe
WMC167
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc167
Xwmc167-2B
wmc167-2B
wmc167
wmc167-2D



Probe for Locus
WMC167
Locus
Xwmc167-2D
[ Show all 6 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 9b2
Donor: Bernard, Clermont-Ferrand
Trimmed to insert at both ends. Multiples inserts (2) in this clone separated 380 -397 by linkers. (CA)22 87 to 130, (CA)8 535 to 550, (CA)8 553 to 568. Primers are for first insert in this clone.
Type
PCR
SSR
PCR primers
Forward AgTggTAATgAggTgAAAgAAg at 7 length 22 bases, Tm 57
Reverse TcggTcgTATATgcATgTAAAg at 170 length 22 bases, Tm 59
SSR size
185 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC167
Source Species
Triticum aestivum